We need everyone interested in plant conservation to rally together for the passage of the Recovering America’s Wildlife Act. With historic levels of funding and support from both sides of the aisle, this bipartisan bill is critical to protecting our nation’s plant life. This rally will feature leaders in plant conservation and give you an opportunity to take action to encourage your Members of Congress to vote YES on this historic bill. Bring your passion and excitement, because we need YOU to push Recovering America’s Wildlife Act over the finish line!
Special guest speakers from Atlanta and California Botanical Gardens, SE Plant Conservation Alliance, Eastern Band of Cherokee Indians, NatureServe, Center for Plant Conservation, Garden Club of America, Office of Kentucky Nature Preserves, & National Wildlife Federation will share the latest information and why we need your help!
The rally will occur November 13th, 2023 from 2-3pm EST. This is a virtual rally hosted on Zoom. Be sure to register here: Meeting Registration – Zoom
Virginia Tech School of Plant and Environmental Sciences; Blacksburg, VA
Country Home Farms; Pembroke, VA
Eastern North America’s tall fescue grasslands produce a plethora of beef stockers, replacement heifers, and cow-calf pairs. These pastures replaced a variety of native woodlands and grasslands in the past 70 years. While tall fescue is a useful forage for much of the year, most tall fescue acreage consists of toxic endophyte Kentucky 31 tall fescue. This endophyte reduces animal performance in terms of average daily gain, reduced reproductive success, and decreased milk production. A lot of this tall fescue acreage cannot be easily converted to alternate forages, such as novel endophyte tall fescue or warm-season forages, in part due to soil conservation as well as economic constraints.
Producers might be able to reduce or eliminate toxic endophyte effects in cattle by overseeding this tall fescue with clovers. Clovers (Trifolium spp) are commonly overseeded into tall fescue stands. Red (T. pratense) and white (T. repens) clovers are currently recommended in Kentucky, Tennessee, and Virginia for pasture clover. There are native alternatives to red and white clover.
A suite of native clovers are present in the United States. Kentucky historically had at least three native species from the Mississippi River to the Appalachian Plateau. These species were present in native grasslands and woodlands throughout the region at the time of European settlement. Native clovers declined with land use change, overgrazing, and fire suppression, but they are still present in modest populations. Native clovers serve a similar nutritional role to wildlife as red and white clovers serve for livestock. Conservation crop science has led to some study as to whether native clovers are agronomically competitive with red and white clover.
Cattle nutrition can justify native clover conservation in pastures
Clovers provide crude protein to cattle in cool-season grasslands. This additional crude protein comes with no additional nitrogen (N) fertilization. Native clovers often serve as the protein concentrate for native ungulates such as bison and deer, as well as monogastric species such as turkeys. Secondary metabolites, such as isoflavones, can dilute or interfere with the alkaloids found in tall fescue pastures. Screening native clover species for traditional metrics of nutritive value can make subsequent analysis for secondary metabolites more effective. Ultimately, this project would provide publishable data regarding the nutritional value of native clovers in comparison to red and white clover under common conditions. This data would guide existing research at Virginia Tech, including Bee-friendly Beef (NIFA-funded). Native clovers are a prime candidate for native plants in livestock production. Including native plants in working grasslands could provide production and conservation opportunities, especially if native clovers offer improved animal nutrition over introduced species.
Native clovers can support cattle and co-exist with non-native grasses
Native clovers are an important protein source in the western rangelands. Species nutritional value has been reported for several of the species native to California, Wyoming, and Oregon range (Bentley & Green, 1954; Cooper, 1957; Cooper and Hunter, 1959; Hamilton, 1961; Hamilton & Gilbert, 1971). These western clovers establish in stands and grow similarly to their nonnative equivalents (Lulow, 2008). Clovers from the eastern United States have only recently been investigated for their agronomic potential. Native clovers have similar agronomic performance relative to traditional red and white clovers.
Image: Jonathan Kubesch
1990s research suggests that native and nonnative clovers cannot be easily crossed, but native species such as Carolina (T. carolinianum) and peanut (T. polymorphum) clovers in addition to the buffalo clovers have improved pest resistance relative to red and white clovers (Taylor et al., 1994; Quesenberry et al., 1997). Running buffalo clover (T. stoloniferum) is competitive in orchardgrass stands subject to hay and forage clipping schedules (Barker and Sparks, 2013). Running buffalo clover also appears to persist in regularly grazed pastures (e.g. Taylor Fork Ecological Area, Eastern Kentucky University; Good Enough Farm, Peru, Indiana). Buffalo clover (T. reflexum) has a similar seed weight and comparable establishment as red and white clovers (Sanne et al., 2023; Kubesch et al., 2023). This work is lacking in reporting the nutritional value of these native clovers.
By Jonathan O. C. Kubesch*,**, Frank Reith*, Dillon P. Golding*,***, Jake Sanne*, Forrest Brown*, Derek Hilfiker*, Joseph D. House****, Jenna Beville*, and Peter Arnold*,*****
*Virginia Tech School of Plant and Environmental Sciences, Blacksburg, VA
**Country Home Farms, Pembroke, VA
***Hoot Owl Hollow Farm, Woodlawn, VA
****Indiana National Guard, West Lafayette, IN
*****Arnold Classic Farms, Chestertown, MD
The public is familiar with red (Trifolium pratense) and white clover (Trifolium repens) growing throughout the Kentucky Commonwealth. However, North America, from Oregon to Florida, is home to a plethora of native clover species. Buffalo clover (Trifolium reflexum) is one of several clover species native to the eastern U.S.A. (Kubesch et al., 2022; Kubesch, 2020). This species demonstrates annual to short-lived perennial life histories, and has potential as a horticultural or agronomic crop (Quesenberry et al., 2003; Kubesch, 2020).
Current efforts to increase native clover populations involve laudable efforts regarding site management, as well as conservation horticulture (e.g Littlefield, 2022). After a site is prepared for planting, plugs are produced. Conservation horticulture work currently executes the following procedure:
Germinate seeds on filter paper in petri dishes (Figure 1)
Transfer seedlings to cell pack trays
Pot up plants into small pots (Figure 2)
Plug individuals into spaced nurseries or maintain on benches for seed production
Figure 1. Running buffalo clover (Trifolium stoloniferum) germinating on filter paper under laboratory conditions. Smyth Hall, Virginia Tech, Blacksburg, VA January 30, 2023.Figure 2. Running buffalo clover (Trifolium stoloniferum) growing in the greenhouse. University Greenhouses Bay 7A, Virginia Tech, Blacksburg, VA February 3, 2023.
In restoration and agronomic contexts, seeding clover has a logistic and resource advantage over plugging clovers. Seeding clover can reduce the need for intensive planting efforts, reduce soil disturbance, and ease transportation of unique plant material. Seeding approaches require a basis for setting a seeding rate and dates. Often, clovers are timed for planting between Valentines’ Day and St. Patrick’s Day in the Upper South. Introduced red and white clovers are commonly frost seeded every several years into cool-season pastures (Kubesch et al., 2020).
Seeding clovers can also take advantage of physiological mechanisms that improve seed establishment. In the field, frost seeding involves defoliation of an existing grassland stand, broadcasting clover seed onto the stand, and letting freeze-thaw cycles incorporate the seed into the soil surface. Compared to many native and introduced grasses, clover seed coats allow the seed to survive freeze-thaw incorporation into the soil surface. Quesenberry et al (2003) reports that buffalo clover has a similar seed weight to introduced clovers. A common rate of pasture frost seeding is 4 lb/A red clover and 2 lb/A white clover (Kubesch et al., 2020).
Optimizing rather than maximizing seeding rate is desirable given the limited seed availability of buffalo clover as well as the desire to increase planting area in restoration attempts. Managers want to get a good stand with as little seed as necessary. In addition to generating stand densities that justify direct seeding over plugging, an optimal seeding rate should generate ground cover that conserves soil as well as meets existing criteria for composition.
The present experiment sought to determine whether a 2 lb/A or a 4lb/A seeding rate can optimize buffalo clover establishment relative to white and red clover. This objective was measured through emergence as well as cover assessments. The hypothesis of this study was that the higher seeding rate will achieve the aforementioned targets comparable to, or greater than, red clover and white clover.
Jake Sanne*, Dillon P. Golding**, Peter Arnold*, Jenna Beville*, Derek Hilfiker*, Forrest Brown*, and Jonathan O. C. Kubesch*, ***
*Virginia Tech School of Plant and Environmental Sciences
**Hoot Owl Hollow Farm, Woodlawn, VA
***Country Home Farms, Pembroke, VA
Introduction
Buffalo clover (Trifolium reflexum), is a rare native clover present in the eastern United States. In Kentucky, this species occurs in the vicinity of Mammoth Cave, as well as further west. Conservation efforts in the eastern United States have maintained many of these native populations, though there is interest in using horticulture and agriculture to increase buffalo clover populations (Quesenberry et al., 2003; Kubesch, 2020; Kubesch et al., 2022).
A limitation of these alternative approaches is the lack of data regarding the establishment of native clovers. For many rare plants, plugs are grown in nurseries and then planted to field sites (Littlefield, 2022). Even in the only published horticultural research, seed was grown into plugs for field plantings (Quesenberry et al., 2003). Buffalo clover has a fair degree of phenotypic variability in growth form, life history, and flower color across the geographic range. These differences in plant material might also suggest diversity in seed characteristics.
Seed weight is an important seed characteristic for increasing buffalo clover populations. Seed weights are associated with increased establishment success (Catano et al., 2022; Westoby, 1998). Published seed weights of red (Trifolium pratense), white (Trifolium repens), and buffalo clover come from a publication using buffalo clover accessions from the Coastal Plain (Quesenberry et al., 2003). Coastal Plain accessions appear to have annual life histories whereas Ohio River Valley accessions appear to be short-lived biennials or perennials (Kubesch, unpublished observations). The present study sought to map and determine seed weights for buffalo clover. The authors hypothesized that Coastal Plain accessions would have different seed weights than the Ohio River Valley accessions.
Materials and Methods
This study consisted of a mapping exercise, seed weight data collection, and then analysis. The mapping exercise discerned the positions of Coastal Plain and Ohio River Valley accessions using the geographic Fall Line as the demarcation between Coastal Plain and Ohio River Valley accessions. Accessions found on the corresponding side of the Fall Line were assigned to a Coastal Plain or Ohio River Valley. This mapping was conducted in ARCGIS software.
Seed weight data for a handful of accessions was accessible from the USDA GRIN system. Notably, these seed weight data overrepresented Coastal accessions rather than Ohio River Valley accessions. Additional data came from Quesenberry et al (2003) as well as manual measurements. Quesenberry et al (2003) selected accessions from TX, GA, MS, and FL. One accession in this study came from the Ohio River Valley. Seed from single plant selections of Cincinnati and Clarks River accessions were measured on a lab balance (Bonvoisin scale).
Data Analysis
Seed weight was treated as a completely randomized design. Initial analysis at the state-level was considered to address potential accession grouping at local scales. Differences in seed weight between accessions from the Coastal Plain and Ohio River Valley were also considered to test other known differences in accessions (e.g. annual or biennial life history). All analyses were conducted in SAS v9.4 (SAS Institute, Cary, NC). PROC GLIMMIX coded for a simple one-way ANOVA.
Jonathan O.C. Kubesch*, Kelly Winklelpleck*, Connor Doyle*, Lindsey Barbini*, John H. Fike*, and Michael Johnson**
*Virginia Tech School of Plant and Environmental Sciences
**Clarks River National Wildlife Refuge
Summary
Buffalo clover (Trifolium reflexum) is a true native clover of eastern U.S. provenance. Previous research suggests that buffalo clover has seed yields and weights similar to introduced clover species (making it suitable for increase). However, such research is limited, with little comparison of plant material from across the northern range of the species. In fact, there is overrepresentation of southern accessions from Georgia, Texas, Mississippi, and Florida. This overrepresentation stems from historical seed collections coming from Coastal Plain collections. In order to better represent accessions from the northern range of the species (both in current research and for future studies), this project sought to 1) conduct a comparison of buffalo clover accessions from across the range against red clover (T. pretense); and 2) bank seed from the Clarks River population for use in conservation and research. Plants were grown from seed to seed and studied in a common garden experiment with efforts taken to maintain genetic purity. Seed was then banked with the Southeastern Grasslands Initiative. Variability in phenotypes across the lifecycle were observed among northern and southern buffalo clover accessions, which suggests that the species complex has further structural variance. Southern accessions required less time to bloom and flowered without vernalization. Clarks River plant material has not yet flowered and appears to require vernalization.
Introduction
The native clovers of eastern North America are relics of the complex mosaic landscapes present prior to European settlement (Gillett and Taylor, 2001). Of these species, buffalo clover is an annual to biennial species with known morphological and reproductive variability (Vincent, 2001).
Kentucky clover (T. kentuckiense) is a recent addition to the flora (Chapel and Vincent, 2013). Species delimitation between Kentucky and buffalo clover has been determined using primarily floral characteristics; however, vegetative characters in a common garden experiment might further resolve the relationships within the species complex.
Previous work with Kentucky clover and running buffalo clover in greenhouse and field research suggest that these native species face fewer propagation challenges in comparison to other rare plants (Kubesch, 2018; Kubesch 2020). Historically, Dr. Norman Taylor at the University of Kentucky maintained all 3 clovers native to Kentucky in greenhouse collections (Kubesch, 2018; Daniel Boone, personal communication).
The objectives of this project were to collect seed from the northern edge of the buffalo clover range and compare buffalo to Kentucky clover. It was hypothesized that Kentucky clover would be more morphologically similar to buffalo clover accessions from Kentucky and Ohio than to accessions from the Coastal Plain.
Join us April 2nd at 11am EDT as we pull winter-creeper from populations of running buffalo clover at Ashland, The Henry Clay Estate in Lexington following the iNaturalist tutorial hike. To learn more about running buffalo clover, click here. You do not need to partake in the iNaturalist tutorial hike to help pull winter creeper, but you are welcome to! Bring a knee pad and gardening gloves if you have them. We will meet next to the Gingko Cafe. If you wish to join in, please email Heidi Braunreiter, heidi.braunreiter@ky.gov.
Wood lily (Lilium philadelphicum), Laurel County, Kentucky. @Littlefield
By Tara Littlefield, Botanist and Plant Conservation Manager
The Office of Kentucky Nature Preserves and partners have been working on a project to protect, connect, and restore populations of the state endangered wood lily (Lilium phildelphicum), and its Pine Barrens and woodland habitat over the past decade in Kentucky. The wood lily, while globally secure and wide ranging, is state endangered in Kentucky despite once being more common. This charismatic plant of the Cumberland Plateau grassland and woodlands (barrens) has declined by over 90% in the past 40 years due to habitat loss, lack of fire, mowing, and herbivory. The wood lily, along with numerous other plants it grows with, make up critical pollinator habitat for species such as the monarch butterfly and native bees. We are working with partners to bring this plant and its habitat back from the brink of extinction by coordinating and implementing monitoring, management and restoration efforts. I am excited to announce that the wood lily has finally come full circle as a plant conservation alliance project from monitoring, collaboration, seed collection, site preparation and management, to now translocation back in managed pine barrens habitat in the Cumberland plateau!
A catalyst for the creation of the Kentucky Plant Conservation Alliance
The conservation efforts for the wood lily represents a shift in plant conservation direction in our state that has happened over the past decade. Funding and targeted conservation efforts have traditionally been focused on globally rare plants, plants that are listed under the Endangered Species Act and have specific federal funding tied to their recover efforts. Since I have worked at Kentucky Nature Preserves in 2005, the lack of staff and funding has always limited the number of projects and species we could reasonably work on outside of our federally listed plant program and state nature preserves site monitoring and management programs. Our efforts for federally listed plants were successful ,and we managed with limited monitoring on our state nature preserves and associated rare species that occurred on these lands. In addition to this work, we had identified so many other plant conservation needs for state listed species across the state that were falling through the cracks due to lack of staff, funding, time outreach and education. Our rare plant records were becoming historic, and the state listed rare plants that we were able to visit on private and public lands were rapidly declining or becoming extirpated since they were originally discovered in the 70s-90s due to various threats. The wood lily is one example of a species that was falling through the cracks.
How do you tackle issue of lack of staff, time and funding to accomplish larger missions beyond the feasible efforts of just a few dedicated staff? What was our approach to addressing these serious plant conservation issues? The key is partnerships and collaboration, and creating an outreach focus of spreading the mission into other organizations and individuals.
Wood lily Conservation History
The majority of the wood lily populations were discovered and documented in the 1970s and 1980s in over 10 counties scattered in the Cumberland plateau region. They were primarily found in powerlines and roadsides and nearby pine oak woodlands and barrens. Over the years, due to habitat loss, lack of fire, summer mowing, and herbivory the populations dwindled down to just a handful of populations. I worked on a project from 2011-2013 updating roadside rare plant populations in the Cumberland plateau region on and near the Daniel Boone National Forest and collected this data. It was alarming. If trends did not change, if threats were not mitigated, we may lose one of our beautiful native lily’s, among many other rare species and habitat, from our state in the near future. With some outreach, new partnerships were formed with KYTC transportation staff and roadside maintenance crews to alter mowing and other management of the rare plant populations. We partnered with David Taylor with the Daniel Boone National Forest and Jim Scheff and Tina Johnson of Kentucky Heartwood on additional monitoring and seed collection efforts. After a failed attempt at seed collection in 2016 due to extreme herbivory, we caged 27 plants scattered in the southern Cumberland plateau region to ensure seed collection in the fall. We knew it was important to propagate and seedbank these plants as soon as possible in order to transplant future plants into suitable habitat in the future. We needed to increase the number of viable populations and make up for some of of the many lost populations over the years.
Jim Scheff of Kentucky Heartwood and Kendall McDonald of OKNP cage wood lily’s to prevent herbivory to ensure seed collection, June 2017. @ T. Littlefield
Seed was successfully collected in 2017 by OKNP, Heartwood, and KNPS volunteers and a new project with Margaret Shea of Dropseed Nursery began. Margaret is a amazing native plant horticulturalist with a rare plant conservation background. Her success at propagating the seeds, growing and safeguarding the plants until we are able to transplant into recipient pine barrens sites is crucial to the restoration and transplantation process.
Margaret Shea @ Dropseed Nursery @H. Braunreiter
At the same time, land managers at the Office of Kentucky Nature Preserves, led by Josh Lillpop, began pine barrens restoration projects at on OKNP state nature preserves and natural areas in order to create more suitable pine barrens habiat for rare plants like the wood lily (Lilium phildelphicum), pine aster (Symphyotrichum concolor) and hairy snoutbean (Rhynchosia tomentosa). We partnered with staff at the Daniel Boone National Forest, including David Taylor, Claudia Cotton, Christy Wampler, and Jacob Royse who helped with population monitoring and connecting with existing pine barrens restoration efforts that could also provide future habitat for the wood lily. While our goal continued to be increasing networking with roadside and utility companies and staff on appropriate management practices for the existing rare pine barrens species, we also strived to create new populations within interior pine barrens restoration sites that are being managed with fire and mechanical removal of canopy, the pine barrens and savannah communities.
In the fall of 2021, 5 years after we began the project to protect the remaining populations and to propagate them for future introductions, the first transplantations finally began! A team from the Office of Kentucky Nature Preserves and Kentucky Plant Conservation Alliance volunteers transplanted wood lily bulbs into new several sites in Powell, Pulaski and Rockcastle counties that are being managed and restored to the pine barrens woodland community.
The November 2021 wood lily bulb planting team consisted of Tara Littlefield, Heidi Braunreiter, Rachel Cook, Vanessa Voelker, Ryan Fortenberry, Lexi Schoenloab, Dale Bonk, Jim Scheff and Tina Johnson. We quickly realized that a great planting tool for these tiny wood lily bulbs were spoons, hence the spoons in some of the group photos. We planted over 500 bulbs across 5 sites with plans to expand on our plantings and sites next year. Monitoring plots were installed and data was collected. Our team will be measuring the success of these plantings over the course of the next year and networking with land managers on future scheduled burns at these sites. Fingers crossed our wood lilies survive and flourish! Stay tuned!
